The application of nitrification inhibitors in soil reduces soil nitrogen loss and improves the utilization rate of nitrogen fertilizer, which is of great significance for environmental protection. However, although we have studied many different kinds of nitrification inhibitors, there are only a few agricultural nitrification inhibitors that can be used in agricultural production, and their applications are not extensive. The two reasons are as follows:

First, nitrification inhibitors are synthetic chemical reagents with certain defects. Nitrification inhibitors can inhibit the activity of nitrifying bacteria in soil, so they should have strong specificity and can not harm the plants and animals and microorganisms in soil. They are not easy to be adsorbed by soil colloid and can move with fertilizer and water. But that's often not the case. For example, too much nitropyridine will poison plants and affect plant roots and leaves. Although C2H2 is more effective as a nitrification inhibitor, it is a gas at room temperature and pressure. The adsorption and fixation capacity of in the soil is weak, so the retention time in the soil is short, and it is difficult to be applied in agricultural production.

Second, the impact of the external environment. There are many factors influencing nitrification inhibitors. Soil temperature, soil texture, soil ph, soil organic matter, nitrogen source and crop species all affect its nitrification inhibition effect. In a word, the application of nitrification inhibitors has certain effect on agriculture. However, most kinds of nitrification inhibitors are not widely used due to their disadvantages in price, technology and pollution. Huang Yizong and other researchers summarized the following reasons: 

① Experimental research effect is very good, but the actual application to agriculture effect is low;

② Due to its own characteristics, some nitrification inhibitors are limited in the promotion and application. For example, acetylene is easy to escape from soil pores, affecting its nitrification inhibition. Some nitrification inhibitors are toxic and have toxic effects on soil flora, fauna and soil microorganisms.

③ Some nitrification inhibitors are toxic and have toxic effects on soil flora, fauna and soil microorganisms.

④ Some nitrification inhibitors are easy to remain in the soil, causing soil pollution.

Therefore, it is necessary for agriculture to search for an environmentally friendly substance that can inhibit soil nitrification.